Fig. 6
From: Poly(a) selection introduces bias and undue noise in direct RNA-sequencing
Poly(A) selection is a source of variability in technical replicates. Cumulative distribution functions of the fold-change RPM between replicates. Each plot is a comparison of two polyA-selected library replicates performed on the same biological sample. The red line in each denotes genes that fell in the most variable decile of tail lengths, shown as red in Fig. 4A (decile 10). Black lines denote all other genes, falling into deciles 1 through 9. A Comparison of two technical replicates produced for this study: Selected-2 and Selected-3. (KS test, decile 10 vs decile 1–9: p-value = 0.00018848) (B and C) Comparison of two sets of poly(A)-selected technical replicate libraries from Roach et al., 2020 [10]; ENA public accession numbers: roach_L3_1: ERR3245468, roach_L3_2: ERR3245469, roach_L4_1: ERR3245470, roach_L4_2: ERR3245471 (Roach L3 set: KS test, decile 10 vs decile 1–9: p-value = 0.00519075. Roach L4 set: KS test, decile 10 vs decile 1–9: p-value = 0.00007283)