Table 1 Selected and Unselected Libraries
From: Poly(a) selection introduces bias and undue noise in direct RNA-sequencing
Library Name: | Bio Sample | Total RNA Mass into Selection | RNA into Library | Flow Cell # | Hours On Flow Cell | Guppy Reads Called | minimap2 Primary Reads Mapped | Reads Assigned to Protein Coding | Bases Called and Mapped |
|---|---|---|---|---|---|---|---|---|---|
Selected-1 | A | 90.0 μg | 464 ng | 7 | 63 hours | 1779.5 k | 1754.6 k | 1097.7 k | 1465 Mb |
Unselected-1 | – | 5.0 μg | 8 | 63 hours | 1345.5 k | 1234.6 k | 820.3 k | 1030 Mb | |
Selected-2 | B | 44.8 μg | 349 ng | 3 | 23 hours | 1388.2 k | 1344.5 k | 909.7 k | 1063 Mb |
Unselected-2 | – | 5.0 μg | 39 hours | 759.8 k | 702.2 k | 484.5 k | 567 Mb | ||
Selected-3 | 90.0 μg | 500 ng | 1 | 67 hours | 2017.1 k | 1972.6 k | 1287.5 k | 1748 Mb |