Fig. 2

Porcine immune cell differential methylation is associated with differential gene expression. A-C Histograms of cDMR methylation-transcript abundance Pearson correlation coefficients across cell types in promoter, intragenic, and intergenic regions, inset by distributions at a random sampling of regions overlapping each feature. p-values indicate the significance in the shift to the left of the cDMR distribution relative to the random distribution as calculated by Wilcoxon rank sum tests. D Enrichment of cDMRs for regions positively and negatively correlated with gene expression, separated by gene feature. * = p < 0.05, ** = p < 1E-3, *** = p < 1E-10 from Fisher’s exact test. E A monocyte and neutrophil lowly methylated region located ~ 19 kb upstream of the SIRPA gene (red asterisk) is significantly negatively correlated with SIRPA abundance. Gray boxes indicate cDMRs, and black dots indicate CpG coordinates. cDMR = cell differentially methylated region