Fig. 2

Percent methylation values for DML created using an euclidean distance matrix. Samples in low pH conditions are represented by black, and samples in ambient pH conditions are represented by gray, with maturation stage along the bottom (0 = indeterminate, 3 = spawn-ready mature female). Darker colors indicate higher percent methylation, and a density plot depicts the distribution of percent methylation values for a panel. After excluding C- > T SNPs, 1284 DML were identified using a logistic regression, using a chi-squared test and 50% methylation difference cut-off