Fig. 7

A The primary culture of branchial cells. B Immunolocalization of NKAα1 (green) in cultured branchial cells. Negative control was incubated with normal rabbit serum. Scale bar = 50 μm. C Analysis of Na+, K+ and Cl− ion transport in branchial cells under hypo- (150 and 250 mOsmol/L) and hyperosmotic (500 and 600 mOsmol/L) stress conditions using an ion chromatography system