Fig. 5

Genomic DNA contributes little to pathway enrichment analysis when comparing two distinct methods. a) Enriched pathways showed a large overlap regardless of gDNA concentration. The Venn diagram shows the number of DEG-enriched pathways compared with Ribo-Zero and Poly (A) Selection. Twenty-five (52.1%) of enriched pathways were shared, regardless of gDNA contamination. b) Most enriched pathways associated with gDNA did not appear in the comparison of Ribo-Zero and Poly (A) Selection. The Venn diagram shows the number of all enriched pathways in the comparison of Ribo-Zero with > 0% and 0% gDNA and Ribo-Zero and Poly (A) selection. The pathway enriched between Ribo-Zero with > 0% and 0% gDNA were considered associated with gDNA. c) Many more DEGs between Ribo-Zero and Poly (A) Selection than between Ribo-Zero libraries. The DEGs were detected by comparing Ribo-Zero libraries (0% to 10% gDNA) and Poly (A) Selection libraries (0% gDNA) and between Ribo-Zero libraries with 10% and with 0% gDNA. PA: Poly (A) Selection; RZ: Ribo-Zero