Fig. 5From: Transcriptome analysis revealed gene expression feminization of testis after exogenous tetrodotoxin administration in pufferfish Takifugu flavidusThe differential expression of piRNAs and mRNA in the MTG and MCG. A-B, Volcano plot showing the differential expression piRNAs (A) or genes (B) in MTG vs MCG, the red dot represents up-regulated genes/piRNAs, the green dot represents down-regulated genes/piRNAs, the gray dot represents no significant difference; C, Relationship between relative expression levels of selected piRNA in the MTG and MCG validated by qRT-PCR and log2 fold changes derived using piRNA sequencing, MCG as a calibrator; D, qRT-PCR and mRNA sequencing of selected genes between MTG and MCG, MCG as a calibrator. Expression levels of piRNA/mRNA measured with qRT-PCR were depicted with column charts, and the red line represents the log2 fold changes/fold changes in sequencing. Error bars represent one standard deviation of three different biological replicates. The internal reference gene is U6 for piRNA and gapdh for mRNA. Set as * P < 0.05, ** P < 0.01 and *** P < 0.001 vs controls. The asterisks represent significant differences between the treatment group and the control group. Statical significance is reported for each piRNA or mRNABack to article page