Fig. 8

Exogenous TTX induces cell apoptosis in the testis of Takifugu flavidus. A, TUNEL staining of Takifugu flavidus gonads after TTX administration. a and c, the ovary of FTG and FCG; b, d, testis of MTG and MCG; The dark brown signals are apoptosis signals by TUNEL staining and the black triangle arrows are indicating the positive signals, the scale bar is 50 μm. B, Apoptosis ratio of gonads in juvenile Takifugu flavidus after TTX administration. Data were shown as mean ± SEM, *, indicating that P < 0.05