Table 1 Equid gammaherpesvirus detection in PBMC-EFK co-cultures by qPCR-HRM targeting the glycoprotein B ORF
Horse no | Host | Single virus positive | EHV2 & 5 positive b | EHV2 & 5 negative c | Isolates sequenced d | HRM Tm °C e | |
|---|---|---|---|---|---|---|---|
EHV2 b | EHV5 b | ||||||
1 | 4 yr old TB a | 8/13 (61%) | 1/13 (8%) | 4/13 (31%) | 0/31 | H1-3–2018 | 86.8 |
2 | 17 yr old TB a | 14/67 (21%) | 17/67 (25%) | 34/67 (51%) | 2/67 (3%) | H2-80–2018 | 89.0 |
3 | 23 yr old pony | 8/16 (50%) | 3/16 (19%) | 2/16 (12%) | 3/16 (19%) | H3-25–2018 | 89.9 |
4 | 34 yr old pony | 8/22 (36%) | 5/22 (23%) | 2/22 (9%) | 7/22 (32%) | H4-147–2018 | 87.0 |
H4-18–2018 | 88.5 | ||||||
5 | Retired TB a | 20/31 (65%) | 0/31 (0%) | 10/31 (32%) | 1/31 (3%) | H5-91–2018 | 88.3 |
H5-57–2018 | 85.0 | ||||||
H5-60–2018 | 89.2 | ||||||
Total | 58/149 (39%) | 26/149 (17%) | 52/149 (35%) | 13/149 (9%) | Â | ||