Whole genome sequence analysis of equid gammaherpesvirus -2 field isolates reveals high levels of genomic diversity and recombination

Table 5 Highly divergent EHV2 genes (Ka values > 0.025)

ORF	Ka ^a	Gene family ^b	Gene conservation ^c	Gene product	Functions
E1	0.139	GPCR 1	EHV2	E1	Intracellular signalling (7TMR domains)
E2	0.089	NA	EHV2	E2	Signalling peptide (Ig domain)
E3	0.130	Complement Control Protein (CCP)	EHV2	E3	Membrane protein
8	0.042	Glycoprotein B	Core	gB	Cell entry and cell to cell spread
E5A	0.090	NA	EHV2	E5A	Unknown
E6A	0.285	E6A	EHV2	E6A	Contain signal peptide
22	0.067	Glycoprotein H	Core	gH	Cell entry and cell to cell spread
46	0.027	Uracil DNA Glycolase (UNG)	Core	uracil-DNA glycosylase	DNA repair
47	0.138	Phage_glycop_gL	Core	gL	Tegument
E7A	0.033	NA	Gamma	g42	Cell entry, binds MHC-II (C-type lectin-like domain)
51	0.208	Epstein-Barr GP350	Gamma	gp350	Cell attachment
52	0.093	BLRF2	Core	virion protein G52
53	0.052	Glycoprotein N	Core	gN	Virion morphogenesis and membrane fusion
64	0.036	large tegument protein	Core	large tegument protein	Capsid transport
E7	0.041	IL-10	EHV2	IL-10	Immune regulation
73	0.086	NA	Gamma	nuclear antigen LANA-1	Involved in latency
74	0.084	GPCR 1	Beta/Gamma	Membrane protein G74	Intracellular signalling (7TMR domains)
E9	0.109	NA	EHV2	Membrane protein E9
E10	0.143	CARD	EHV2	apoptosis regulator E10	Involved in apoptosis

^a Ka = nonsynonymous substitutions per nonsynonymous site
^b NA = not assigned to a gene family
^c Gene conservation over different herpesvirus subfamilies: Core = conserved in all herpesviruses, Gamma = conserved in all members of the subfamily gammaherpesvirinae, Beta/Gamma = conserved in all members of the betaherpesvirinae and gammaherpesvirinae subfamilies, EHV2 = EHV-2-specific gene

ISSN: 1471-2164