Fig. 8

Plants overexpressing AgBZR1.5 exhibited BR-responsive phenotypes. A Phenotypes of light-grown 3-week-old Arabidopsis Col-0 overexpressing AgBZR1.5 with a YFP tag (AgBZR1.5-OX/Col). B The differential accumulation pattern of AgBZR1.5 protein was detected using an anti-GFP antibody. The upper and lower bands indicated the phosphorylated and unphosphorylated forms of AgBZR1.5 proteins, respectively. CBB (Coomassie brilliant blue G-250) staining was used to assess protein loading. Full-length blots/gels were presented in supplementary Fig. S1. C The expression analysis of BR-repressed genes (DWF4 and CPD) and the BR-induced gene (PRE5) in the leaves of 3-week-old AgBZR1.5-OX/Col transgenic Arabidopsis plants under 1 μM eBL immersion for 1 h. Asterisk (*) denotes P < 0.05, as determined by a Student’s t-test