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Table 1 Amplification efficiency of standard curves in candidate reference genes and target gene

From: Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

Gene symbol

Efficiency (%)a

Slope

R2 values

ACE2

94.12

−3.471

0.999

B2M

96.64

−3.405

1.000

β-actin

90.30

−3.579

0.999

GAPDH

95.32

−3.439

0.997

HMBS

92.59

−3.513

0.948

HPRT1

97.19

−3.391

0.999

18S

93.01

−3.502

0.998

  1. a PCR amplification efficiency is calculated by the following formula: Efficiency = (10–1/slope-1) × 100
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BMC Genomics

ISSN: 1471-2164

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