Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

Table 2 Descriptive statistics and pairwise correlation of the six reference genes analyzed by BestKeeper algorithm

	B2M	b-actin	GAPDH	HMBS	HPRT	18 s
n	105	105	105	105	105	105
geo Mean [CP]	18.52	15.71	19.57	23.34	23.45	16.88
AR Mean [CP]	18.56	15.74	19.61	23.43	23.46	16.9
min [CP]	15.43	13.93	16.54	18.12	21.34	14.86
max [CP]	21.43	18.63	21.56	26.09	24.98	18.94
std dev [+/− CP]	0.99	0.75	0.89	1.49	0.59	0.62
CV [% CP]	5.31	4.74	4.54	6.37	2.54	3.66
min [x-fold]	−8.52	−3.43	−8.19	−37.36	−4.32	−4.07
max [x-fold]	7.51	7.53	3.97	6.73	2.9	4.15
std dev [+/− x-fold]	1.98	1.68	1.85	2.81	1.51	1.54
Pearson correlation coefficient (r)
BestKeeper vs.	B2M	b-actin	GAPDH	HMBS	HPRT	18 s
coeff. of corr. [r]	0.536	0.479	0.054	0.698	0.625	0.479
p-value	0.001	0.001	0.584		0.001	0.001

Abbreviations: n number of samples, geo Mean [CP] the geometric mean of CP, CP crossing point, AR Mean [CP] the arithmetic mean of CP, min [CP] and max [CP] the minimum and maximum values of CP, std. dev [+/− CP] the standard deviation of the CP, CV [% CP] the coefficient of variance expressed as a percentage on the CP level, min [x-fold] and max [x-fold] the minimum and maximum values of expression levels expressed as an absolute x-fold over- or under-regulation coefficient, std. dev [+/− x-fold] standard deviation of the absolute regulation coefficients [14]

ISSN: 1471-2164