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Table 3 Expression stability values of candidate reference genes in all samples

From: Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

Gene

BestKeeper

Delta CT

Genorm

Normfinder

RefFinder

 

Rank

SD

Rank

SD

Rank

MV

Rank

SV

Rank

GM

HPRT1

1

0.59

1

1.31

1

1.02

1

0.38

1

1.00

18S

2

0.62

2

1.41

2

1.02

2

0.70

2

1.68

β-actin

3

0.75

3

1.47

3

1.11

3

0.89

3

3.00

GAPDH

4

0.89

5

1.81

5

1.38

5

1.48

5

4.73

B2M

5

0.99

4

1.61

4

1.21

4

1.17

4

4.23

HMBS

6

1.49

6

2.08

6

1.62

6

1.85

6

6.00

  1. Abbreviations: SD standard deviation, MV M-value, SV stability value, GM geometric mean [17]
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BMC Genomics

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