Table 6 Reference genes used in this study and primer
Gene symbol (gene name) | GenBank Accession number | Primer forward (F)/reverse (R) | Amplicon size |
|---|---|---|---|
ACE2 (angiotensin converting enzyme 2) | NM_001123070.1 | F: TGCCAGGGCCAAATGGT R: ATCTAGTGGATACGTTTTGGCAAT | 67 |
B2M (beta-2-microglobulin) | NM_213978.1 | F: CGGAAAGCCAAATTACCTGA R: CATCTTCTCCCCGTTTTTCA | 88 |
β-actin (beta-actin) | XM_021086047.1 | F: GGATGCAGAAGGAGATCACG R: ATCTGCTGGAAGGTGGACAG | 130 |
GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) | NM_001206359.1 | F: CCACCCAGAAGACTGTGGAT R: AAGCAGGGATGATGTTCTGG | 81 |
HMBS (hydroxymethylbilane synthase) | DQ845174.1 | F: AGGATGGGCAACTCTACCTG R: GATGGTGGCCTGCATAGTCT | 83 |
HPRT1 (hypoxanthine phosphoribosyltransferase 1) | NM_001032376.2 | F: AAGCTTGCTGGTGAAAAGGA R: GTCAAGGGCATAGCCTACCA | 100 |
18S (18S ribosomal RNA) | NR_046261.1 | F: CCCACGGAATCGAGAAAGAG R: TTGACGGAAGGGCACCA | 122 |