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Fig. 2 | BMC Genomics

Fig. 2

From: Reducing costs for DNA and RNA sequencing by sample pooling using a metagenomic approach

Fig. 2

Comparison of DNA-seq data for pooled and non-pooled samples. Dickeya dadantii percentage deviation between the local chromosomal coverage (5 kb window) of different sample combinations. R2 values of the compared local chromosomal coverage are indicated above individual columns. Escherichia coli percentage deviation between the local chromosomal coverage (5 kb window) of different sample combinations. R2 values of the compared local chromosomal coverage are indicated above individual columns. Vibrio natriegens percentage deviation between the local chromosomal coverage (5 kb window) of different sample combinations. R2 values of the compared local chromosomal coverage are indicated above individual columns. D Scheme of gene copy numbers caused by overlapping replication rounds. E Marker frequency analysis of exponentially growing Dickeya dadantii cells. Colors of the data points indicate the two replicates. F Marker frequency analysis of exponentially growing Escherichia coli cells. Colors of the data points indicate the two replicates of pooled samples. G Marker frequency analysis of chromosome I of exponentially growing Vibrio cholerae cells. Colors of the data points indicate the two replicates of pooled samples. H Marker frequency analysis of chromosome II of exponentially growing Vibrio cholerae cells. Colors of the data points indicate the two replicates of pooled samples. I Copy number effect between oriC and the terminus region during exponential growth derived from pooled and single samples. The ordinate represents the fold change of copies of oriC or oriV relative to the terminus region

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