Fig. 9
From: Comprehensive analysis of RNA m6A methylation in pressure overload-induced cardiac hypertrophy
The correlation between m6A modification and mRNA expression of KCNN2 and BMP4 in hypertrophic mice hearts. A MeRIP-qPCR analysis of the m6A modification on KCNN2 mRNA after four weeks of TAC (n = 6 each); B qRT-PCR analysis mRNA expression level of KCNN2 after four weeks of TAC (n = 4 each); C-D MeRIP-qPCR analysis of the m6A modification on BMP4 mRNA after four weeks of TAC (n = 4 each); E qRT-PCR analysis mRNA expression level of BMP4 after four weeks of TAC (n = 5 each); F Representative immunoblots of BMP4; G Densitometry quantification of protein (n = 3 ~ 5 each); H-I Gravimetric analysis of heart weight normalized to body weight (HW/BW) and lung weight normalized to body weight (LW/BW) after eight weeks of TAC (n = 6 each); J-K Measurements of internal dimension at end-systolic (LVID.s) and left ventricular posterior wall thickness at end-diastole (LVPW.d) after eight weeks of TAC (n = 4 each); L-M MeRIP-qPCR analysis of the m.6A modification on BMP4 mRNA after eight weeks of TAC (n = 3 each); N: qRT-PCR analysis mRNA expression level of BMP4 after eight weeks of TAC (n = 5 each). The results are expressed as the means ± SD (*p < 0.05, compared to the sham group)