Table 1 Primers used for qRT-PCR
From: Comprehensive analysis of RNA m6A methylation in pressure overload-induced cardiac hypertrophy
Genes | Primer types | Primer Sequences (5’-3’) |
|---|---|---|
METTL3 | Forward | CGCTGCCTCCGATGTTGATCTG |
METTL3 | Reverse | CTGACTGACCTTCTTGCTCTGCTG |
METTL14 | Forward | TGCAGCACCTCGGTCATTTA |
METTL14 | Reverse | TAACCCCACTTTCGCAAGCA |
WTAP | Forward | GAAGGAGACACGACAGCAGTTGG |
WTAP | Reverse | GCTTGTGACCTCTGCCTGATCTAC |
FTO | Forward | ATGAAGACGCTGTGCCACTGTG |
FTO | Reverse | CACGTTGTAGGCTGCTCTGCTC |
ALKBH5 | Forward | GCAAGGTGAAGAGCGGCATCC |
ALKBH5 | Reverse | GTCCACCGTGTGCTCGTTGTAC |
GAPDH | Forward | GGTTGTCTCCTGCGACTTCA |
GAPDH | Reverse | TGGTCCAGGGTTTCTTACTCC |