Fig. 9

Verification of transcriptome results by qRT-PCR. Six genes related to ABA, JA, and rice defense were selected, and relative expression levels were determined by qRT-PCR, and compared with transcriptome results. Genes included the following: (A) OsABA8ox2 encoding ABA 8’-hydroxylase (LOC_Os08g36860); (B) OsAOS1 encoding allene oxide synthase (LOC_Os03g55800); (C) OsJAZ11 encoding jasmonate ZIM-domain protein (LOC_Os04g32480); (D) OsPYL9 encoding pyrabactin resistance-like abscisic acid receptor (LOC_Os06g36670); (E) OsBZIP23 encoding bZIP transcription factor (LOC_Os02g52780) l; and (F) Osbph6 encoding leucine rich repeat family protein (LOC_Os04g35210). Error bars represent means ± SE (n = 3); three or more independent experiments were conducted. Columns with different letters indicate significance at P < 0.05 using Duncan´s multiple range test