Table 1 RT-qPCR primers of mRNA for cumulus cells and Granulosa cells
Gene | Transcript ID | Primer sequences (5′-3′) | Product Size (bp) |
|---|---|---|---|
RAC1 | ncbi_102273253 | F: CCCAACACACCCATCATC R: TCGCCTCATCAAACACTG | 202 |
CDKN1A | ncbi_102268672 | F: ACTTGGACCTGTCGCTGT R:GGAGTGGTAGAAATCTGTCAT | 131 |
VEGFA | ncbi_102270607 | F: GGCTGCTGTAATGACGAA R: TCTCCTATGTGCTGGCTT | 104 |
ITGB1 | ncbi_102273972 | F: AGACGACTTGGAGAATGTG R: GCTGGTGTTGTGCTAATG | 135 |
SGK1 | ncbi_102272039 | F: AGTTGTTCTACCATCTCCAG R: CGTTGTGCCATTGTGTTC | 215 |
FGFR4 | ncbi_102267813 | F:CCACCACATTGACTACTACA R: TCCAAACGACCACACATC | 118 |
JAK3 | ncbi_102286011 | F: CGGATGATGGGATGTGAG R: AATGATGGTCGGTCTTGAG | 158、 |
NR4A1 | ncbi_102288141 | F: GGAGGTGATTCGCAAGTG R: CCAGACGGAGGATAAAGAG | 113 |
ITGA6 | ncbi_102265941 | F:AAGATGATATGGATGGAGGAG R: AACGCAATGTAATGGAAGTC | 124 |
LAMA5 | ncbi_102266736 | F: GTGGTGTCCTTGGTGAAC R: GCTGATGTCCTTGATACTGT | 195 |
AKT3 | ncbi_102265427 | F: GCCTTGGACTATCTACATTC R: CCCGCACATCATTTCATAC | 255 |
CCND1 | ncbi_102270546 | F: CGAGGAGAACAAGCAGAT R: GCGGTGATAGGAGAGGAA | 172 |
β-actin | NM_173979.3 | F: CCGTGACATCAAGGAGAAG R: AGGAAGGAAGGCTGGAAG | 174 |