Fig. 4

The distribution of fragments per kilobase of transcript per million (FPKM) values and visualization of differentially expressed mRNAs in adult and juvenile groups. A A Comparison of the up-regulated and down-regulated mRNAs B The Volcano plot of mRNA differential expression in adult and juvenile scorpions. Red and blank points refer to those differentially and not differentially expressed genes compared to adult scorpions (logFC ≥ 2 or logFC ≤  − 2 and p-value < 0.01). The scatter plot is a visualization method used for showing differentially expressed. C The heatmap of hierarchical clustering analysis. Heatmap showing the comparison of the gene abundance in the two groups of adult and juvenile. Colors from purple to yellow represent the gene expression abundance from downregulation to upregulation, respectively