Fig. 3

The FPKM values and relative expression levels of nine randomly selected DEGs in alfalfa seeds at five development stages. Bars indicate the level of relative expression based on the qRT-PCR results (left Y-axis). Plots show the transcript abundance change (FPKM) based on the results of the RNA-Seq analysis (right Y-axis). Alfalfa seeds were obtained at 11, 19, 27, 35, and 43 days after pollination, and the time points were designated as S1, S2, S3, S4, and S5, respectively