Fig. 11

Binding of WRKY70 to the sequence S2 requires the WT-boxes CGACTTTT. Lanes 1: free probe. Lanes 2: free probe plus purified protein extract of E. coli not expressing WRKY70. Lanes 3: free probe plus purified WRKY70. Lanes 4: free probe, purified WRKY70, and unlabelled competitor (S1 or S2, respectively) in a 4000-fold molar excess. Lanes 5: free probe, purified WRKY70, and unlabelled mutations S1mut1 or S2mut1, respectively in a 4000-fold molar excess. The sequences of S1, S1mut1, S2, and S2mut1 are shown. Unchanged nucleotides in S1mut1 and S2mut1 are indicated by a dash (−). A P designates the position of the free probe and a specific DNA-protein complex is marked with an asterisk (*). The gel shifts were done three times and one example is shown. The original unprocessed figures are shown in Fig. S3 and Fig. S4