Fig. 3

The four proximal WT-box containing sequences from the ATEP3 upstream region confer WT-box-dependent reporter gene expression. Transient reporter gene assays in parsley protoplasts after transformation and treatment with and without Pep25 (light grey bar and dark grey bar, respectively). The reporter plasmids used for transformation harbour four copies of the indicated sequences upstream of the uidA (GUS) reporter gene. Relative GUS expression (pmol 4-MU/min/mg protein) and standard deviations were determined from eleven (pBT10, D) or three (S1, S1mut1, S2, S2mut1, S3, S3mut, S4, S4mut1, S5, S5mut1, S6, S6mut1, S7, S7mut1) independent experiments with technical duplicates, respectively. Statistical differences between experiments were determined with a t-test in microsoft excel. Statistically significant Pep25 inductions are indicated with one (p < 0.05), two (p < 0.01), and three (p < 0.001) asterisks. Sequences of the seven monomers (S1 through S7) and the seven sequences in which the WT-box are mutated are shown. The WT-box core sequence GACTTT is marked in bold. Altered nucleotides in the mutations are shown and unaltered nucleotides are not shown (−)