Fig. 4

WT-box core sequence adjacent nucleotides contribute to MAMP-responsive gene expression of the four proximal WT-boxes from the ATEP3 upstream region. Transient reporter gene assays in parsley protoplasts were done as described in Fig. 3. Relative GUS expression and standard deviations were determined from twelve (pBT10, D), nine (S4), seven (S1, S2), four (S2mut3, S3, S3mut2, S3mut3), or three (S1mut2, S1mut3, S2mut2, S4mut2, S4mut3) independent experiments with technical duplicates, respectively. Statistical differences between experiments were determined with a t-test in microsoft excel. Statistically significant Pep25 inductions are indicated with asterisks as described in Fig. 3. Sequences of the four monomers (S1 through S4) and the eight sequences in which the WT-box core sequence adjacent nucleotides are mutated are shown. The WT-box core sequence is marked in bold. Altered nucleotides in the mutations are shown and unaltered nucleotides are not shown (−)