Fig. 8

WRKY50 negatively regulates MAMP-responsive reporter gene expression through WT-box containing synthetic promoters. Transient reporter gene assays in parsley protoplasts after co-transformation of the effector plasmid WRKY50-pORE or the empty vector (pORE) with reporter plasmids harbouring four copies of the indicated sequences upstream of the uidA (GUS) reporter gene. Relative GUS expression and standard deviations were determined from six (pBT10, D, pBT10 + pORE, pBT10 + WRKY50pORE), twelve (S22 + pORE, S22 + WRKY50pORE), or three (S1 + pORE, S1 + WRKY50pORE, S2 + pORE, S2 + WRKY50pORE) independent experiments with technical duplicates, respectively. Statistical differences between experiments are indicated with one (p < 0.05), two (p < 0.01), and three (p < 0.001) asterisks. The sequences of S1 and S2 are shown in Fig. 3