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Fig. 2 | BMC Genomics

Fig. 2

From: Using single-worm RNA sequencing to study C. elegans responses to pathogen infection

Fig. 2

Testing three conditions for the mechanical lysis of worms. Thirty worms per group were mechanically lysed under three different conditions (Table 1), followed by RNA extraction using an RNeasy Mini Kit. A RNA concentrations were measured using a Qubit fluorometer. Each bar in the histogram represents the mean value of three replicates. Error bars represent standard deviation. B RNA integrity was assessed using a Fragment Analyzer and is expressed as an RNA Quality Number (RQN). An RQN of 10 equates to a perfect RNA sample without any degradation products, and an RQN of 1 indicates a completely degraded sample. Numbers in-between are used to indicate progressing degradation states of the RNA sample. Each bar in the histogram represents the mean value of three replicates. Error bars represent standard deviation. An asterisk (*) denotes a significant difference (p < 0.05) between two conditions. C Representative profile of RNA extracted under Condition 1. RFU stands for Relative Fluorescence Units. D Representative profile of RNA extracted under Condition 2. E Representative profile of RNA extracted under Condition 3

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