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Fig. 4 | BMC Genomics

Fig. 4

From: Using single-worm RNA sequencing to study C. elegans responses to pathogen infection

Fig. 4

Quality metrics of single-worm and standard RNA libraries. A Alignment rates were calculated as the percentage of reads mapped to the C. elegans reference genome out of the total passing-filter reads. Each bar in the histogram represents the mean value of five libraries. Error bars represent standard deviation. B Alignment distributions of single-worm and standard RNA libraries were calculated as the percentages of reads mapped to the coding, UTR (untranslated region), intronic, and intergenic regions out of the total passing-filter reads. Each bar in the histogram represents the mean value of five libraries. Error bars represent standard deviation. C The coverage of various positions of transcripts by mapped reads is shown. Each transcript was subdivided evenly into 1000 bins. Each curve represents the average of five libraries. D rRNA content was calculated as the percentage of reads mapped to rRNA sequences out of the total passing-filter reads. Each bar in the histogram represents the mean value of five libraries. Error bars represent standard deviation. The asterisk (*) denotes a significant difference (p < 0.05) between the single-worm RNA libraries and the standard RNA libraries. E Duplication rates were calculated as the percentage of reads that were duplicates out of the total passing-filter reads. Each bar in the histogram represents the mean value of five libraries. Error bars represent standard deviation. * denotes a significant difference (p < 0.05) between the single-worm RNA libraries and the standard RNA libraries

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