Fig. 5

Concordance of expression quantification within single-worm and standard samples. A Representative scatter plot of the log₂(cpm + 1) values of standard libraries (Standard_01 vs. Standard_02) and Spearman’s rank correlation coefficient for the two libraries. B Representative scatter plot of the log₂(cpm + 1) values between single-worm libraries (Single_worm_01 vs. Single_worm_02) and Spearman’s rank correlation coefficient for the two libraries. C Heatmap of the sample-to-sample distances of single-worm and standard libraries via hierarchical clustering. Dark blue to light blue represents close to distant clustering. D Principal component analysis (PCA) of single-worm and standard libraries using log₂(cpm + 1) values. Open blue, filled blue, open orange, and filled orange dots represent uninfected single-worm, infected single-worm, uninfected standard, and infected standard libraries, respectively