Fig. 6

Pathogen infection-induced differential gene expression detected by single-worm and standard RNA-seq. A A pairwise scatter plot of the log₂ ratio values of the 21,453 genes commonly detected by both single-worm and standard RNA-seq. The plot was fitted with a linear regression line, and the equation and R² value are shown. B Venn diagram showing the numbers of DEGs significantly changed at least 1.5-fold that were identified by single-worm RNA-seq only, by standard RNA-seq only, and by both methods (overlapping DEGs). C A pairwise scatter plot of the log₂ ratio values of the 1,590 overlapping DEGs. The plot was fitted with a linear regression line, and the equation and R² value are shown. D A pairwise scatter plot of the log₂ ratio values of the 9,332 non-overlapping DEGs. The plot was fitted with a linear regression line, and the equation and R² value are shown