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Fig. 3 | BMC Genomics

Fig. 3

From: A conserved transcriptional fingerprint of multi-neurotransmitter neurons necessary for social behavior

Fig. 3

CGN phenotype is constant throughout development. Expression of cholinergic and GABAergic markers between 1 and 4 dpf. A Transverse section at 1 dpf showing expression of cholinergic (ChAT) and GABAergic (GABA) immunolabeling. A’, A”, B′, B″, and C′, C″. Rectangular boxes in A’ define selected areas, shown at higher magnification in A”, containing neurons labeled with the respective image color. Red arrows label vTely321 neurons, cyan arrows cholinergic vTely321 neurons, and yellow arrows label GABAergic vTely321 neurons. Merged images showing vTely321 neurons labeled (white arrows) that are cholinergic and GABAergic from 1dpf to 4dpf. A”, B″ and C″ inserts from areas defined in A’, B′ and C′. Arrows in merged images A’, B′, and C′ and inserts A”, B” and C” show selected vTely321 neurons that are cholinergic and GABAergic. B, C Coronal sections of ventral telencephalon showing cholinergic and GABAergic markers in vTely321 neurons at 2 and 4 dpf. (Scale bar for all images = 20 μm). D Boxplots showing the percentage of vTely321 neurons that are CGNs at 2 and 4 dpf

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