Fig. 4
LIMTF genes are expressed throughout development. A-C Wholemount chromogenic ISH in 20 hpf embryos showing expression of lhx8a, lhx6 and isl1 (left to right). Arrows label the principal foci of LIMTF gene expression in the brain. Magenta arrows denote telencephalic clusters and white arrows denote diencephalic clusters respectively at that timepoint. islet1 is more broadly expressed compared to lhx8a and lhx6. D-F 24 hpf larval forebrain transverse sections showing LIMTF gene expression in vTely321 neurons. D lhx8a mRNA expression in vTely321 neurons. E lhx6 mRNA expression in vTely321 neurons. F islet1 protein expression in vTely321 neurons. G-I. Coronal sections of the adult ventral forebrain showing (left to right) expression of lhx8a, lhx6 and isl1 in vTely321 neurons. Selected neurons that express the representative transcripts are denoted by white arrows. (Scale bars: A-C = 50 μm; scale bar in R’ represents D, D′, E, E’, F, F′, G, G’, I, I′ = 30 μm; scale bar in F″ represents D″, E”, F″, G”, H″, I″ = 30 μm)