Fig. 5

Expressions of candidate lncRNAs during beige adipocyte differentiation. A Beige adipocyte differentiation protocol of ASCs and 3T3-L1 cells. B ASCs and 3T3-L1 cultured with thermogenic adipogenic medium were stained with Oil Red O to determine the level of adipogenesis. Scale bar: 100 µm. C qPCR analysis of mRNA encoding Ucp1, Pgc-1α, and Cidea at different time points (day 0, 1, 3, and 5) during ASCs differentiation. D Protein expression of UCP1 detected by Western blots at different time points (day 0, 1, 3, and 5) during ASCs differentiation. E qPCR analysis of AK029592, humanlincRNA1030 and ENSMUST00000152284 lncRNA expressions at different time points (day 0, 1, 3, and 5) during ASCs differentiation. F qPCR analysis of mRNA encoding Ucp1, Pgc-1α, and Cidea at different time points (day 0, 1, 3, and 5) during 3T3-L1 differentiation. G Protein expression of UCP1 detected by Western blots at different time points (day 0, 1, 3, and 5) during 3T3-L1 differentiation. H qPCR analysis of AK029592, humanlincRNA1030 and ENSMUST00000152284 lncRNA expressions at different time points (day 0, 1, 3, and 5) during 3T3-L1 differentiation. All data were presented as mean ± s.d. (n = 3)