Fig. 1From: De novo annotation of lncRNA HOTAIR transcripts by long-read RNA capture-seq reveals a differentiation-driven isoform switchHOTAIR expression during adipogenic differentiation. a Quantitative RT-PCR analysis of HOTAIR expression in HeLa, MCF7, MDA-MB-231, and ASCs from two independent donors (ASC-1 and ASC-2). b Differential expression of HOTAIR between differentiation time points analyzed by short-read RNA-seq (mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001, limma moderated t-statistic; n = 3). c RT-qPCR analysis of HOTAIR relative expression, normalized to D0 ASCs, during adipogenic and osteogenic differentiation (mean fold difference ± SD; ****p < 0.0001, two-way ANOVA; n = 3)Back to article page