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Fig. 3 | BMC Genomics

Fig. 3

From: De novo annotation of lncRNA HOTAIR transcripts by long-read RNA capture-seq reveals a differentiation-driven isoform switch

Fig. 3

HOTAIR splicing across functional domains during adipogenesis. a Schematic representation of HOTAIR 3’ exons (exons E4 to E7) containing LSD1 and PRC2 binding domains. Alternative splicing events and polyA (pA) sites usage detected in 5 representative HOTAIR high confidence isoforms are represented. b Proportion of long-read Capture-Seq reads for each E7 length across differentiation time points. c Schematic representation of RT-qPCR amplicons (upper panel) and qRT-PCR analysis of HOTAIR E7 length variation during adipose differentiation (lower panel). d PacBio Capture-Seq analysis of HOTAIR exon E5 alternative splice site usage and e of the proportion of major exon E5 splice variants during adipogenic differentiation. f Schematic representation of PCR amplicons (upper panel) and semi-quantitative RT-PCR analysis of HOTAIR expression using primers located in exons E3 and E5 (E3-E5; lower panel). SF3A1 is shown as a loading control. RT-: no reverse transcriptase control; NTC: no template control. Full-length gels are presented in Additional file 4, Fig. S4

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