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Fig. 5 | BMC Genomics

Fig. 5

From: De novo annotation of lncRNA HOTAIR transcripts by long-read RNA capture-seq reveals a differentiation-driven isoform switch

Fig. 5

HOTAIR isoform expression is highly cell-type specific. a Number of SRA samples with 10 or more junction spanning reads for exons E3.1-E4 and E3-E4. b Semi-quantitative RT-PCR analysis of HOTAIR expression in ASCs, primary myoblasts, BJ fibroblasts, HEK293T and HeLa cells using primers located in HOTAIR exons E3.1-E4 and E3-E5. SF3A1 is shown as a loading control. Full-length gels are presented in Additional file 4 Fig.S7. c IGV tracks over HOTAIR locus showing predicted HOTAIR regulatory elements (REMs) from the EpiRegio database [42, 43] (red: activating, blue: repressing), average ATAC-Seq tracks from ASCs [44, 45], Myoblasts [46] and HeLa cells [47], and ChIP primers location. d ChIP-qPCR analysis of histone modifications during adipogenesis of ASCs (mean ± SEM of n ≥ 3 independent differentiation experiments; **p < 0.01 Mixed effect analysis)

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BMC Genomics

ISSN: 1471-2164

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