Fig. 2

Relative expression levels of NlSPARC in N. lugens, as determined by real-time quantitative PCR (RT- qPCR). A Expression patterns of NlSPARC in different developmental stages including E-1d ~ E-6d (eggs from 1 to 6 days), N-1d ~ N-12d nymphs from 1 to 12 days, F-0d ~ AF-4d, newly emerged females, 2- and 4- days old adult females; M-0d ~ AM-4d, newly emerged males, 2- and 4- days old adult males. B Expression patterns of NlSPARC in various tissues including FHD, head from females; FTX, thorax from females; FAM, abdomen from females; MHD, head from males; MTX, thorax from males; MAM, abdomen from males; MIRO, internal reproductive organ of males; FIRO, internal reproductive organ of females; GT, guts; SG, salivary glands; HM, hemolymph; LG, legs; WT, wings and teguments. All data are presented as means ± SE with three biological replicates and relative expression levels of target genes were calculated by RT-qPCR with the 2^‒ΔΔCT method. Different lowercase letters above the bars indicate significant differences among different tissues by the Tukey’s multiple range test at P < 0.05