Fig. 4From: SPARC plays an important role in the oviposition and nymphal development in Nilaparvata lugens StålEffects of dsNlSPARC on fecundity (A), survival rate of progeny (B) and expression level of NlSPARC in N. lugens (C). The 5th instar nymphs, newly emerged adults and 4-day old gravid females were injected with dsNlSPARC respectively. Individuals treated with dsGFP were used as a control. The number of offspring and egg was calculated from fifteen biological replicates (Mean ± SE). NlSPARC mRNAs levels of 30 new hatched nymphs and 20 10-day old mixed insects with 10 females and 10 males were analyzed by RT-qPCR with the 2‒ΔΔCT method. Data is presented as mean ± SE with five biological replicates. G1NHN, newly hatched nymphs from F1 progeny; G1 10d, 10d-old nymphs from F1 progeny. Different lowercase letters and ** above the bars indicate significant differences at P < 0.05 and P < 0.01 respectively among different treatments by the Tukey’s multiple range tests.Back to article page