Table 3 Primer sequences and restriction enzymes for genotyping
From: PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
Polymorphism | Sequence (5′ → 3′) | Tm (°C)b | Enzyme | Size (bp) |
|---|---|---|---|---|
PMEL p.Leu18del | F: GGAAGGAAGAACAGGATGGATCT R: TAGGGAGAGAAAAACCAGAGCAG | 55 | MboII | Leu: 108, 40, 18 del: 146, 18 |
 p.Gly22Arg | F: ACTGTCAATGAGTAGCAGGATGTC R: TGCACCCAAATCTTCATGTG | 60 | SfcI | Gly: 244, 190 Arg: 434 |
 p.Ser36Leu | F: AAGCCACAACTACCTGAC R: TAGGCCCATCATTGCTGAC | 60 | Af1II | Ser: 492 Leu: 266, 226 |
 p.Ala612Glu | F: AGCCAGGATCAAGACCAAG R: GATAGCTGTTAAGTAAGTGG | 60 | BlpI | Ala: 319, 144 Glu: 463 |
LYST p.His2015Arg | F: GAAAATTACAGCAGAAGTCCTTGG R: TGACAAACATAAGTATTAGTAGGAGG | 60 | FokI | His: 66, 42 Arg: 108 |
 p.Ala2575Val | F: TACAGATTCACTTCAGTCGCCTGCTa R: TCTTGGAGGAAAATCTTCTCAAACACTAT | 60 | Fun4HI | Ala: 184, 24 Val: 208 |
USP13 p.Met1Val | F-1st: TCGCCATTGGATTAAAAATAGCAGCGT R-1st TACCGGGGAGTCGTAGGAGAAGG F-2nd: CAGCGTCCCCTTCACCAG R-2nd ACTCGTTCTTGTAGACCCTG | 1st: 65 2nd: 60 | EagI | Met: 268, 65 Val: 149, 132, 65 |