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Fig. 2 | BMC Genomics

Fig. 2

From: High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes

Fig. 2

Experimental design used for insertion site detection in Arabidopsis and Camelina For each transgenic line, a total of 10 PCR reactions were performed with five corresponding to the 3’ and 5’ ends of the respective transgenes, or to the left and right border. Separate reactions were performed for all pairwise combinations of a left- or right-border and one of four genome-walking primers (denoted with a “PST” prefix) and indexed separately prior to sequencing. One representative reaction (represented here with PST primers 1-4) was pooled prior to indexing to determine if the insertion site signal was strong enough to be detected in a single reaction

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