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Table 2 Summary of BLAST results obtained for each line and PCR library

From: High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes

 

Number of BLAST hits per PCR library

 

Line

Total input reads

Border

PST1

PST2

PST3

PST4

Pooled

Total hits

Line #1415

1,187,867

LB

7545

262

35

139

3158

11,139

RB

35

16

54

0

9

114

Line #1416

1,593,516

LB

5577

6578

753

57

2426

15,391

RB

33,208

395

98

600

7327

41,628

GK-269g12

625,724

LB

0

0

11

16,923

10,391

27,325

RB

17

20

24

32

18

111

WT

189,053

LB

10

0

64

0

7

81

RB

0

4

9

0

0

13

  1. The total number of reads reflects the number of informative reads remaining for each line after mapping to the T-DNA vector, removing T-DNA matching bases, and applying read length filtering. BLAST hits represent the number of reads passing the query search parameters and resulted in a corresponding match to the plant genome. Number of hits are shown for each sequencing library separately and total hits summarized across all libraries for the left- and right-border