Fig. 2

Effects of lncMYOZ2 knockdown on adipocyte differentiation. A Expression of lncMYOZ2 in porcine preadipocytes treated with siRNA mix for 36 h. B, C Adipogenic phenotypes of lncMYOZ2-knockdown or control porcine preadipocytes, after induction of adipogenic differentiation, as assessed by ORO staining (B) and triglyceride accumulation (C). The mRNA (D) and protein (E) levels of CEBPα，PPARγ and FABP4 at day 8 post-induction of adipocyte differentiation, as detected by qRT-PCR and Western blot. F The mRNA levels of lncMYOZ2-overexpression in porcine preadipocytes. G, H The adipogenic phenotypes of porcine preadipocytes transfected with lncMYOZ2-pCMV-N-FLAG or control after adipogenic induction for 8 days were assessed by ORO staining and triglyceride accumulation. I, J qPCR and Western blot analysis of CEBPα，PPARγ and FABP4 at day 8. Data are shown as mean ± SEM. * (P < 0.05) and ** (P < 0.01) indicate significant differences compared to control