Genome sequence of the entomopathogenic Serratia entomophila isolate 626 and characterisation of the species specific itaconate degradation pathway

Table 11 Primers used in this study

Primer	Sequence (5’-3’)^a	Amplicon size (bp)	Amplicon
ItaF ItaR	aaatctagaGGTTTGTACCCGCCGTTAGCAG aaatctagaCTCGCCCTTGACGGCCTGATCG	3677	Ita-RipC
tetNcoI_f tetNcoI_r	aaaccatggGAGTTAGTCTTGAAGTCATGCGC aaaccatggGCATTCACAGTTCTCCGCAAG	1611	Tetracycline cassette
rip_f rip_r	gatatcCAGATCATCGAATCCCACCGT gatatcGTGGTTGGCGCATCTCCC	1443	ripC
M13F M13R	GTAAAACGACGGCCAGT GCGGATAACAATTTCACACAGG

^a Lower case denotes the addition of a poly-A tail where restriction enzyme sites are underlined

ISSN: 1471-2164