Fig. 5
From: Structural and developmental dynamics of Matrix associated regions in Drosophila melanogaster genome
Validation of MARs and effect of transcription inhibitors on MARs. Southern blot validation for seven 5’-UTR MARs chosen on the basis of sequencing data. C1 and C2 are control MARs present in stabilized, unstabilized or RNase A treated NuMat. U1 to U5 are unique MARs present only in stabilized NuMat. The upper picture in each panel (a to e) shows the EtBr-stained gel profile of PCR amplified MAR sequences from genomic DNA. The lower picture shows the blot probed with 32P labelled MAR DNA obtained from NuMat isolated under condition as indicated. The C1 and C2 MARs are NuMat associated under all circumstances, whereas U1 to U5 MARs are gained after stabilization and lost after RNase A treatment. Their intensity reduces after actinomycin-D treatment and is completely lost after triptolide treatment. The gels and the blots have been cropped from top and bottom to fit into a single panel for easy comparison