Table 2 Assembly statistics and completeness assessment (by BUSCO against Eukaryota and Nematoda databases) of the de-novo H. bacteriophora transcriptome assembly

 Number of transcripts

46,599

 Total bases (bp)

95,741,839 (95.7 Mb)

 Minimum sequence length (bp)

501

 Maximum sequence length (bp)

19,436

 Average sequence length (bp)

2,054.59

 N50 value (bp)

2,681

 (G + C)s

38.11%

Assembly completeness assessment using BUSCO

Against eukaryota_odb10.2019–11-20 (Total BUSCO groups = 255)

 Complete BUSCOs (C)

243

95.30%

 Complete and single-copy BUSCOs (S)

75

29.40%

 Complete and duplicated BUSCOs (D)

168

65.90%

 Fragmented BUSCOs (F)

5

2%

 Missing BUSCOs (M)

7

2.70%

Against nematoda_odb10.2019–11-20 (Total BUSCO groups = 3,131)

 Complete BUSCOs (C)

2,930

93.60%

 Complete and single-copy BUSCOs (S)

1,109

35.40%

 Complete and duplicated BUSCOs (D)

1,821

58.20%

 Fragmented BUSCOs (F)

50

1.60%

 Missing BUSCOs (M)

151

4.80%