Fig. 4

Robust BMP Signaling is initiated in Explants Around the Onset of Gastrulation. A Western blot analysis of lysates of developing epidermal (WT) and neural (20uM K02288) explants for pSmad1/5/8 and Smad1 with Actin loading control. Gel was cropped to minimize empty space, uncropped gel is Supplemental Fig. 8A. B Significance of BMP overrepresentation (hypergeometric p-value) in temporally differentially expressed genes. C-D Graphs of BMP responsive genes in epidermal and neural lineages (C) Ventx2.1 (D) Id3. Graphs are sums of S + L allele. Width of the line represents SEM of three biological replicates