Fig. 6

Time Series Data Provides Novel Insights into Mesendoderm GRN. A Heatmaps of genes in the published mesendoderm GRN across time in the endoderm and ventral mesoderm lineage with colors representing z-score of TPM expression in both lineages [106]. (B) Schematic of the timing of genes from published Mesendoderm GRN in both the endoderm and ventral mesoderm lineage, as defined by expression of at least 30 TPM in the L and S allele combined for the average of three biological replicates. C TPM of Bix1.3, Evx1, and Mix1 revealing mesoderm onset dynamics. D-G TPM of genes proposed as novel mesendoderm GRN members based on DESeq2 and Limma analysis (D) Tmcc1 (E) Nptx2 (F) Ca14 (G) Pygm. Graphs are sums of S + L allele. Width of the line represents SEM of three biological replicates