Fig. 7
Early BMP signaling drives ventral mesoderm rather than early epidermal divergence. A Western Blot Analysis of lysates for developing epidermal (WT) and ventral mesoderm (BMP4/7 20 ng/uL) explants for pSmad1/5/8 and Smad1 with Actin loading control. Gel was cropped to minimize empty space, uncropped gel is Supplemental Fig. 8B. B Number of differentially expressed genes between successive developmental stages of the epidermal and ventral mesoderm lineage, padj ≤ 0.05. C Venn Diagrams for the total number of DE genes between stages for the epidermal and ventral mesoderm lineages, as well as the number of genes increasing and decreasing over time. D-I TPM of genes representative of different expression dynamics in response to early BMP (D) Grhl1 (E) Ventx2.1 (F) Post (G) Vegt (H) Wnt8a (I) Mix1. Graphs are sums of S + L allele. Width of the line represents SEM of three biological replicates