Skip to main content
Fig. 8 | BMC Genomics

Fig. 8

From: Quantitative analysis of transcriptome dynamics provides novel insights into developmental state transitions

Fig. 8

BMP Signaling is Restrained Until stage 10.5 by Dand5 Activity. A-B TPM of genes involved in BMP signaling (A) BMP heterodimer ligands BMP7.2, BMP4, BMP2 and primary BMP target Smad, Smad1 (B) maternally provided BMP antagonists Dand5 and Gtpbp2. Graphs are sums of S + L allele. Width of the line represents SEM of three biological replicates. C Western Blot Analysis of lysates for stage 10 epidermal (WT), Dand5 MO injected (40 pmol/embryo), ventral mesoderm (BMP4/7 20 ng/uL), and endoderm (160 ng/uL Activin) explants for pSmad1/5/8 and pSmad2 with Actin loading control. Gels were cropped to minimize empty space, uncropped gel is Supplemental Fig. 8C. D qRT-PCR of animal pole explants examining the fold change from stage 9 to 13 of expression of mesodermal marker Bra(T) for epidermis(WT), ventral mesoderm(BMP4/7 20 ng/uL) and Dand5MO(300pmo/embryo) injected(***P < 0.005)

Back to article page