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Table 1 Summary of founder screening data

From: A robust pipeline for efficient knock-in of point mutations and epitope tags in zebrafish using fluorescent PCR based screening

Gene

Somatic screening data

Germline transmission data

# of founder fish screened

# of founder fish positive

# of founder fish positive / # of founder fish screened

# of positive F1 pools / # of total F1 pools

# of positive F1 embryos / # of total F1 embryos

# of positive F1 adults / # of total F1 adults

tcnba

133

13

2/12

4/11

3/16 (18.8%)

9/45 (20.0%)

9/16

2/16 (12.5%)

4/40 (10.0%)

gata2b

41

7

2/7

3/7

6/32 (18.8%)

26/96 (27.1%)

4/24

2/58 (3.4%)

2/164 (1.2%)

gba

95

22

4/11 (1 precise, 3 with errors)

3/21

11/94 (11.7%)

10/96 (10.4%)